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Comparison of Different Culture Methods on Bacterial Recovery in Hemodialysis Fluids


Napawan Punakabutra BSc*, Pongpun Nunthapisud MSc*,
Trairak Pisitkun MD**, Khajohn Tiranathanagul MD**,
Kriang Tungsanga MD**, Somchai Eiam-Ong MD**

* Department of Microbiology, Faculty of Medicine, Chulalongkorn University
** Department of Medicine, Faculty of Medicine, Chulalongkorn University


To examine the culture method that could provide the highest bacterial recovery, 143 reverse osmosis water samples used in hemodialysis were collected for comparison of the media (Tryptic Soy Agar, TSA vs Reasonerís 2A Agar, R2A), the temperature (20°C vs 37 °C), the duration of incubation (48-hour vs 7-day), and the culture technique (membrane filtration vs spread plate methods). The European Best Practice Guideline method, R2A at 20°C for 7-day incubation provided higher bacterial recovery than the Association for the Advancement of Medical Instrumentation (AAMI) method, TSA at 37°C for 48-hour incubation. The membrane filtration method gave better yield than the spread plate method. As such, the European Best Practice Guideline method in combination with the membrane filtration technique would be the culture method of choice for hemodialysis fluids.

Keyword : The european best practice guideline, The association for the advancement of medical instrumentation, Tryptic soy agar, Reasonerís 2A agar

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