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Modified GC Medium for Identification of Group B Streptococci


Somchai Santiwatanakul, PhD*, Chantana Mekseepralard, PhD**,
Wongsawat Sawatpunyachote, BSc (MT)***, Sarayuth Susasin, BSc (MT)***

* Department of Pathology, Faculty of Medicine, Srinakharinwirot University
** Department of Microbiology, Faculty of Medicine, Srinakharinwirot University
*** Medical technology student at Faculty of Align Health Science, Chulalongkorn University


The modified GC medium (MGC) was developed for identification of beta-hemolytic group B streptococci. This medium was developed on the basis of enhancing–pigment production of group B streptococci. Three hundred and thirty isolates were tested including 180 isolates of beta-hemolytic group B streptococci, 102 isolates of beta-hemolytic non-group B streptococci, and 48 isolates of Enterococcus faecalis. All isolates of group B streptococci gave carotenoid pigment by this medium. On the other hand, all of non-group B streptococci and E. faecalis did not show pigment after 72 h incubation. The specificity and sensitivity of MGC was 100 %. There were no false positive and false negative in this medium. The MGC may be the alternative of choice for the presumptive identification of group B streptococci.

Keyword : MGC, Carotenoid pigment, Group B streptococci, GBS, Streptococcus lactiae, beba-hemolytic streptococci

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